Visual Phasing is a technique whereby the DNA of siblings is assigned to each of the four grandparents. It is usually done with 3 siblings, though you can adapt the technique, as I did, with 2 siblings and a nephew. As I mentioned in that post, I knew I want to try it as soon as I heard about it. I wasn’t really sure whether it would be helpful for me, but I was curious about the technique. While I was waiting for my sister’s and nephew’s results, I was chatting with a genealogist I know who referred to Visual Phasing as a “party trick”. She didn’t feel that there was much to be gained from doing it.
Having now mapped most of my chromosomes using Visual Phasing, I respectfully disagree – at least for me. Your mileage may vary.
Following are a couple of ways that Visual Phasing has helped me:
1. I can easily identify whether a match is on a line of interest
The vast majority of my DNA matches are on my maternal grandmother’s side, as this line has deep Colonial American roots. As discussed in a previous post, I’m currently particularly interested in matches on my maternal grandfather’s side, as that’s where I’m attempting to break down a brick wall.
When I get a new match on GEDMatch, MyHeritage or FTDNA, I can quickly and easily figure out which side the match is on. For example, on GEDMatch, when I get a new match, I run a “Multi Kit Analysis”, select “Manual Kit Selection/Entry”, enter the new kit number in the 1st box, then compare it against my mother (FC), me, my sister (JK) and my nephew (RM):
I click on Visualization Options, and select 2-D Chromosome Browser
For this match, I see that on Chromosome 11, she or he matches my mother (so I know it’s on my maternal line), matches R from 115 to 129, and matches me (L) from 119 to 129. I compare this to the phased chromosome:
As you can see by the the section outlined in red, this must be a Prowse match (purple). Since that’s my mother’s paternal side, the line I’m particularly interested in, I add this match to my spreadsheet as a match of interest. Doing this systematically has helped me develop a subset of matches to work with.
2. I have a much better understanding of how DNA is passed down.
When I first got my DNA results and started working with matches, there was a lot I didn’t quite get, like:
Why, with some matches, do I share the exact same amount as my mother, with some I share about half, and with others still I don’t share any at all?
Why do I match some people that my sister doesn’t, and vice versa?
How can my nephew share more with a person than I do, when he’s one generation farther back from the match than I am?
I have since learned that these are extremely common questions. I quickly learned that the stock answer is “because of the randomness of how DNA is inherited”. It was only when I started doing visual phasing that I really got it. It makes sense to me now. For example, with the match above my mother and my nephew (her grandson) share about the same amount with this person, I share a bit less, and J is not a match at all. And that’s totally normal.
It took seeing it for me to really understand.
I know that not everybody can do Visual Phasing. If you don’t have siblings to work with, you’re out of luck. But if you do, it’s worth considering. Of course, only you can decide if there’s a value to you – it depends on what you’re trying to accomplish.
For me, it was worth it. Plus, it’s kinda fun – but then again, spreadsheets and graphics make me happy.
What do you think? Party trick, or valuable tool in the genetic genealogy toolbox?
I’ve always loved logic puzzles, so when I first heard about Visual Phasing as a way to determine which segments of my DNA I got from which grandparent, I knew I had to try it. Problem is, you need three siblings to do Visual Phasing, but as my oldest sister died over ten years ago, I only have two siblings to work with. But I do have a nephew, my oldest sister’s son. When I saw a blog post on Genealogy Lady about VP with 2 siblings, I figured I should be able to adapt the process to working with 2 siblings and a nephew. Besides, I also have my mother’s DNA results, so that would be a big help.
Both my sister and nephew agreed to be tested. While I waited for their results to come in, I started practising using a friend’s results, along with his two siblings. I did enough to get a feel for how it worked. That was very helpful before trying to adapt it to my situation.
Here’s the process I followed. I’m assuming that you understand the basics of Visual Phasing, so I won’t be explaining every step – only the ones that differ from the standard 3-sibling approach. See here for the basics, and if you’re going to attempt Visual Phasing, I highly recommend you join the Visual Phasing group on Facebook. I used Steven Fox’s fabulous Excel Visual Phasing spreadsheet (available through the Facebook group).
Here’s the setup:
L – me
J – my sister
R – my nephew
Paternal grandfather – Cooper (orange)
Paternal grandmother – Sharpe (blue)
Maternal grandfather – Prowse (purple)
Maternal grandmother – Hicks (green)
In the setup, I included my mother in the cousin table. While this wouldn’t be of much use with 3 siblings, since all would share a complete chromosome with her, including her is very useful for comparing to my nephew R (her grandson). While I don’t have any 1st or 2nd cousins to work with, I have a few 2C1Rs on my Cooper side, and lots of 3rd-4th cousins on my Hicks side. I have very few known cousins on my Sharpe or Prowse lines (at least not that have uploaded to GEDMatch, or anywhere else with a chromosome browser).
Figure 1: I started with my mother in the extra view, and set the crossover points
Note that in addition to the crossovers that you see in the top section, there’s an additional one for R compared to his grandmother (outlined in red). I included that as well, even though it doesn’t appear when comparing R against L or J.
Figure 2: I added the megabase values and assigned the crossovers.
Red circles – these crossovers clearly belong to R
Blue circles – the first one is J, 2nd is L
Green circles – these ones are either L or J, but I can’t tell which
Purple circles – the crossovers at either end are confusing. I’ll leave them unlabelled for now and come back to them later.
Figure 3: I shaded out the unnecessary parts of R’s chromosome.
This is where it is different using a nephew instead of a sibling or a even a half sibling. When I first did this, I shaded out the whole paternal (upper) chromosome, since I only share R’s maternal chromosome with him (he’s my sister’s son). In the maternal (lower) chromosome, I used all four grandparents. When his mother (my sister) passed on her chromosome to him, recombination would mean that he would get segments from all 4 of her grandparents (his great-grandparents).
The spreadsheet gave me an error message every time I had more than two grandparents represented on the same line. I posted in the Visual Phasing Facebook group asking how I could turn off the warning. Someone suggested that I do it this way instead, which worked much better.
So in Figure 3 (above), where the bottom line is blacked out, it means that R received his mother’s paternal chromosome and there is no match to his maternal grandmother (seen in the extra view). Where the top line is blacked out, R received his mother’s maternal chromosome, as can be seen in the extra view.
Figure 4: Here’s where we start assigning segments. I found it easiest to always start with R, and since I knew whether the segment was maternal or paternal, I didn’t need to use G1, G2, G3, G4 at all, and could start straight away with P1, P2 and M1, M2.
I picked a maternal segment of R’s, and labelled it M1. Since L&R match and J&R don’t, L=M1 and J=M2. And since L&J don’t match on that segment at all, I set the paternal segments to L=P1 and J=P2.
Figure 5: I could then extend to the next crossover points.
Figure 6: There’s a crossover on either side of R’s M1. On the right, it clearly goes from M1 to M2. On the left, it switches from M to P. Since he matches L and not J, it must be P1.
On the far left, there’s a crossover at 2.6. Before that crossover, R matches both L and J, but L and J don’t match each other. Could it be that L and J do match, but it doesn’t show up with the GEDMatch defaults of 7cM and 500 SNPs?
I ran a GEDMatch comparison of L & J and dropped the thresholds to 3cM and 300 SNPs:
Sure enough, L & J do match at the beginning (and also at the end, we’ll come to this later). Therefore, the crossover at 2.6 must belong to J (Figure 7), and it’s a crossover on the paternal side.
Figure 8: At 36, there’s a crossover that I already established belongs to J. Since we know that R is maternal at this point, and J&R changes from matching to not matching at this point, then this must be a maternal crossover, where J changes from M2 to M1.
Figure 9: At 44.5, R changes from maternal to paternal (don’t forget, that’s not his paternal, it’s his mothers. We’re not dealing with his paternal chromosome at all). Since he matches L, it must be P1. Then, at 82, it changes from P1 to P2, and stays the same until R’s next crossover.
Figure 10: At 92.7 and 107, there are crossovers belonging to L or J, but we don’t know which. It’s not on the paternal side, as there’s no change in the match of either to R. But which is it? Does L change from M1 to M2, or does J?
We’re stuck. Without knowing which one changes here, we can’t go any further. Nor can we know whether R’s next section is M1 or M2. Time to start bringing in some cousins. Maybe that will help.
Figure 11: First up, is George, my 2C1R on my Cooper (paternal) side. Since he matches L but not J, we now know that P1 = Cooper (orange) and P2 = Sharpe (blue).
Figure 12: Next up, is E. Hicks, a 3C1R on my Hicks (maternal) side.
Because she matches in the new section, we know that the maternal side there is Hicks. Note that if you’re using Stephen Fox’s Excel spreadsheet to do this, you will get an error if you have more than 2 entries on the same line (like here, where I have M1, M2 and Hicks). You just need to click OK, and you can keep going.
Although it looks like the match between L & E. Hicks extends farther on the right than J, if you look at the numbers, they both end at 113,620,851.
However, since J’s match only begins at 107, we now know that that crossover must belong to J. As well, we can do some extending to the right (Figure 13).
But what about the crossover at 92.9 (outlined in red)? It could be an L crossover, which would mean that M1 = Prowse. Or it could be a J crossover, which would mean that M1=Hicks. Which is it?
Fortunately, I have another known 4th cousin match on Chr. 11 (along with a larger segment on another chromosome). I hadn’t included him when I entered cousins into the spreadsheet, so I ran a GEDMatch Multi Kit Analysis on GW, FC (my mother) and the three of us.
G.W. is a match on the Prowse side. Therefore, we know that M1=Prowse and thus the crossover at 92.9 belongs to L (Figure 14). So M2=Hicks.
Figure 15: The crossover at 119.2 belongs to L. And since there’s R and L match after that point, it must be a maternal crossover from Hicks to Prowse.
Which brings us to the final crossover, after which it appears that nobody matches anyone else. However, it’s impossible for R to match neither L or J, if L & J don’t match each other. There must be some matching at a lower threshold.
L & J:
L & R: No match
J & R:
Therefore, we need a crossover that will result in L=J, L≠R, J=R
If the crossover belongs to L (paternal), then L=J, but L=R and J≠R
If the crossover belong to L (maternal), then L=J and L≠R, but J≠R
If the crossover belongs to J (paternal), then L=J, but L=R and J≠R
If the crossover belongs to J (maternal), then L=J and J=R, but L=R
If the crossover belongs to R, then J=R and L=R, but L≠J
I may like logic puzzles, but that just broke my brain. Maybe there are two crossovers. Maybe one of those matches is real and one isn’t. In any case, I looked at each of our matches on GEDMatch’s Matching Segment Search tool (Tier 1), and none of us have any matches at that part of the chromosome (at least not at the default thresholds), and it’s a very small segment (3.8cM) so I’m not going to worry about that little bit at the end, at least for now.
So, the last step is to merge the segments, and here we have it:
Or to just look at the chromosomes:
You can see that on the paternal side, I (L) received a complete Cooper chromosome, whereas J received an almost complete Sharpe one. R’s mother got both. We all have some recombination on the maternal side.
Chromosome 11 was a fairly easy one to figure out (with the exception of that end bit), since I had some good cousin matches to work with. In a later post, I’ll share some of the more challenging ones I faced. As well, I’ll do a post on how VP has helped me in my research.
Until then, if you have any thoughts or questions on this, or if you need any clarification, please leave a comment and I’d be happy to respond.
I was going to call this post “Breaking down a brick wall with DNA”, but decided that would be false advertising. I’m hoping that eventually this will become a case study (to go along with my case study on Using mtDNA for Genealogy), but first I have to actually break down a brick wall. But I’m nothing if not optimistic, so consider this a case study in progress!
As do most of us, I have a number of brick walls. Over the past few years, I’ve intermittently worked at some them, with varying degrees of success. I’m hoping that DNA can help me break through at least a few. I did my first autosomnal DNA test at the beginning of 2015, with Family Tree DNA (FTDNA). Since then, I’ve also tested with AncestryDNA, have tested my mother on both FTDNA and Ancestry, and have tested a couple of other family members on Ancestry. I’ve uploaded our results to GEDMatch and MyHeritage.
To date, I’ve taken a somewhat scattershot approach to working with my DNA results. I get easily distracted and start down one path, only to then chase a new shiny down another. I’ve made a decision. It’s time to…
I’ve decided to pick a brick wall and chip away at it systematically until it crumbles. I’m going to focus on John William Kirkland and Elizabeth Weeks (okay, that’s two brick walls, but they were a couple, so it makes sense to work on both of them). I wrote about Elizabeth Weeks in my first 52 Ancestors post. John W. Kirkland will likely be the focus of a future post. John and Elizabeth were the grandparents of my great-grandmother, Wilhelmina (Minnie) Kirkland.
I’ve picked them for a few reasons:
I have a 2nd cousin (also a great-grandchild of Minnie Kirkland) and a 2nd cousin, once removed (a grandchild of Minnie’s brother, Lebert) who have tested on Ancestry. These matches help me identify shared matches that are Kirkland/Weeks descendants
I have a critical mass of matches on this line. To date, by using shared matches and building out some people’s trees, I have identified 12 family groups who descend from 4 of their 9 (or so) children.
I have a great collaborator on this research, a 4th cousin who is also a Kirkland/Weeks descendant – Andrew McKnight (who is also a very talented musician – check out his website!).
I have already done a lot of traditional genealogy research on this family, so I have a good starting base. Most of this has been documented on the WikiTree profiles of John William Kirkland and Elizabeth Weeks and their children.
Here’s what I know
The predominant theory about the Kirkland/Weeks family found around the internet seems to all come from an Encyclopedia Titanica profile of Charles Leonard Kirkland, who died on the Titanic. This article includes the following:
Charles Leonard Kirkland was born in March of 1841 in Miramichi, Northumberland County, New Brunswick, the fourth child of John V. Kirkland and Elizabeth Sarah Weeks. The Weeks family had emigrated to New Brunswick from England circa 1820 and John Kirkland, a silk merchant, had emigrated to New Brunswick from Glasgow, Scotland in the early 1830’s. Following their marriage, they moved frequently between New Brunswick and Prince Edward Island, as John built up his importing business. Charles spent his early years in Miramichi where his older brothers, John (born in 1832), James (born in 1835) and William had also been born and raised.
The family relocated to Summerside, Prince Edward Island in 1845, where the first daughter of the family, Lavinia Rebecca, was born in 1849 and the youngest child, Emma Lydia, was born in 1855.
Breaking this down:
John V. Kirkland…a silk merchant, had emigrated to New Brunswick from Glasgow, Scotland in the early 1830s.
I believe John’s middle name was William
I’ve found no evidence that he was a silk merchant. Everything I have found points to him being a cabinet maker
I’ve found no evidence that he emigrated from Scotland in the 1830s. Every source I have found has him born in New Brunswick (maybe Boiestown?), probably around 1802 (though sources vary). Perhaps his father came from Glasgow?
Elizabeth Sarah Weeks. The Weeks family had emigrated to New Brunswick from England circa 1820…
I’ve found nothing that gives a middle name of Sarah
I’ve found no evidence that she emigrated from England around 1820. Anything I’ve found on her children that lists their mother’s place of birth has either New Brunswick, Prince Edward Island or the United States. Not England.
Following their marriage, they moved frequently between New Brunswick and Prince Edward Island, as John built up his importing business. Charles spent his early years in Miramichi where his older brothers, John (born in 1832), James (born in 1835) and William had also been born and raised.
They did move between NB and PEI, as they had children born in both provinces, though I don’t think it was to build up an importing business.
I’ve confirmed sons John (my 2g grandfather) and James, but I’ve found nothing on a William. My 2ggf’s name was John W. Kirkland – I’m assuming the W. is William, like his father. Was there a son William as well?
The family relocated to Summerside, Prince Edward Island in 1845, where the first daughter of the family, Lavinia Rebecca, was born in 1849 and the youngest child, Emma Lydia, was born in 1855.
There was an older daughter, Rebecca Melvina, b. 1834 in New Brunswick. She married in 1854 and died in 1857.
Lavinia (seen in records as Leavinia) was probably born about 1844. There is a New Brunswick late registration of birth stating she was born in 1849, but considering she had her first child in 1860, 1844 is more likely. Her birth record was created in 1929, when she was about 85, so it’s not a surprise that the year is wrong.
Emma Lydia was indeed born in PEI, likely in 1855 (sources vary).
Most of the Encyclopedia Titanica article focuses on Charles’s life as an itinerant preacher in Maine. I’ve found lots of evidence that corroborates this part of the story (and it is a really interesting read!)
However, I’m still not sure about why he went to Glasgow (which ultimately lead to his booking passage on the Titanic to get home). The article states:
He left Tuxford in November of 1911 to sail to Glasgow, Scotland to settle the estate of his fathers two brothers, who were reputed to have owned a business in Glasgow which was to be inherited by the John V. Kirkland family in America. Charles was commissioned by the family to settle the estate in the interest of the entire family.
The article includes a photo of a letter sent by Charles to his daughter from Glasgow, which includes the line “I have not yet found any of Uncle’s brothers and if I can’t find them I won’t be able to get the money”.
I’ve tried to use this information to make a connection to Kirklands in Glasgow, but so far, to no avail. There was a Kirkland who died in Glasgow in September 1911, but he was far too young to have been an Charles’s uncle. I’ll write more about this in a later post.
What I’ve done to date
Visual Phasing, so that I can identify which of my mother’s matches on GEDMatch come from her paternal side;
Started reviewing other match’s trees to look for common surnames/locations. So far, Woodworth and Blakely are names that keep popping up, but I’ve not yet determined if there’s a link.
Now that I’ve identified a number of matches who are descendants of John Kirkland and Elizabeth Weeks, other people who multiple known descendants, or who match on a DNA segment identified as having come from this couple, are either a) also Kirkland/Weeks descendants or b) descendants of an ancestor of either John Kirkland or Elizabeth Weeks. This latter group could help me break through the brick walls.
Here’s what I need to do now:
Contact all AncestryDNA matches that I’ve identified as being on my mother’s paternal side to request that they upload to GEDMatch (or MyHeritage or FTDNA or anywhere with a chromosome browser!);
Contact Kirkland/Weeks matches with no trees to see if they a) have a tree elsewhere or b) can provide me with sufficient information to build out a tree;
Explore tools to identify common surnames/locations in a more systematic way;
Go through MyHeritage and FTDNA matches to identify other Kirkland/Weeks matches;
Contact relevent GEDMatch matches to see if they have trees I can view.
Determine what target testing might be useful;
Look for any opportunities to add yDNA or mtDNA to the testing mix;
In addition to using DNA, I’ll continue with traditional genealogical research. Specifically, I’d like to try to track down the sources used in the Encylopdia Titanica article, to see if this might help reconcile the differences I’ve found.
I plan to blog about the various techniques I’ve already used and my future research, so if this interests you, stay tuned. I’ll use the category “Chipping Away at a Brick Wall” for anything related to this research.
If you’re a descendant of John William Kirkland and Elizabeth Weeks and you’ve done a DNA test (or plan to), please do let me know and I would be most appreciative if you would upload your results to GEDMatch (there are step by step instructions here). Even if you don’t match me or my mother, you may match some of the other known descendants. Even if you have not done a DNA test, I’d love to hear from you. You just may have the piece of the puzzle we need!
Part 4 – Comparing results: I compared my test results to those of the two testers and found a match between me and the descendant of Barbara Richardson.
Part 5 – Conclusion: this post
Now that we know that Charlotte and Barbara were sisters, this opens up new avenues to pursue to determine who their parents were. So what are the possibilities?
Well, first up, there’s the story that Charlotte’s father was a British Army Officer whose regiment was stationed in Charlottetown, PEI. Well, it turns out that the earliest British Regiment on Prince Edward Island was established in 1783, 7 years after Charlotte’s birth and one year after Barbara’s. However, there was a militia called “Callbeck’s Provincial Company” from 1776 to 1783. So it could be that Charlotte’s father was a local resident who was part of the militia. Unfortunately, we’ve yet to locate a listing of militia officers to confirm this.
Early British records from Prince Edward Island show that there were three Richardsons on the Island in the late 1700s, all in Charlottetown – James, George and Alexander.
Many people have Barbara Richardson’s parents as Alexander Richardson and Ann Whittingham. They were married in England in 1779 and arrived in Charlottetown in 1780, two years before Barbara’s birth in 1782. However, if Charlotte and Barbara were sisters, we can rule them out as their parents, since Charlotte was born in 1776 on PEI, a full four years before Alexander and Ann arrived.
So that leaves James or George. While we don’t yet have conclusive evidence, James seems to be the more likely candidate. For one thing, the earliest Island record for George Richardson is a land grant in 1779, whereas there are records for James as early as 1770, including a land grant in 1771.
And there are a couple of other facts that, while circumstantial, are consistent with James and Hannah being the parents of Charlotte and Barbara:
Both Charlotte and Barbara had daughters named Hannah.
James Richardson died in 1782 or 1783. Hannah then married David Dobson. David Dobson was the older brother of Richard Dobson. At the time of David and Hannah’s marriage, Richard was 15 and Charlotte was 8. It is not at all inconceivable that Richard and Charlotte met as youth, when Richard’s brother married Charlotte’s mother, and that they would marry 10 years later, when Charlotte was 18.
If indeed James and Hannah were Charlotte’s parents, we still don’t know where they were born or who their parents were. But we now have some avenues to explore that we didn’t have before. Could Hannah be from West Sussex, where my perfect match’s ancestor was born? It’s certainly a possibility. As for James, there’s evidence to suggest that James, George and Alexander Richardson were closely connected – maybe they were brothers? We’re still working on this.
In the first post of this series, I talked about DNA testing as fishing trips and hunting trips. mtDNA is not the ideal tool for a fishing trip. Even if you have perfect matches in the database, your common ancestor may well be too far back to be identified. But don’t discount mtDNA for genealogy! As I hope you’ve seen from this example, given the right conditions, mtDNA can be a very useful tool.
You have to be dealing with an all-female line. If there are any men between you and the person you’re interested in, your mitochondrial DNA is not going to help you. You’ll need to find a female-line descendant of that ancestor to test.
You have to have a theory to test. If all you know about your ancestor is her first name, mitochondrial DNA is not going to be very useful. You might get lucky and have a match that provides a clue, but it’s a longshot.
You need to be able to find female line descendants to compare to. Ideally, I’d love to test a descendant from Hannah’s second marriage to David Dobson in order to confirm that Hannah was Charlotte’s mother, but unfortunately, they are only known to have had sons. Without a daughter whose line I could follow, there’s nobody to compare to. That is, until we determine her last name – maybe we’ll get lucky and discover a sister with some living descendants.
Overall, I think it’s pretty exciting that we’ve been able to use DNA to discover information about people who lived over 200 years ago. And while we haven’t definitively answered the question of who Charlotte Richardson’s parents were, we’re a lot closer than we were, thanks to mitochondrial DNA.
I’d love to hear your thoughts on this case study. I hope it was helpful!
Welcome to the fourth post of this series, where we get to the fun stuff – comparing results! In the first post, I introduced my 5th great grandmother, Charlotte Richardson and the two competing theories as to who her parents were. In the second post, I outlined the process I followed to identify possible matches, in order to test the two theories. In the third post, I contacted female-line descendants and found one from each line who agreed to take the mtDNA test.
To determine Charlotte Richardson’s haplogroup, along with the haplogroup of all of her children and her daughters’ children, and so on down the line, I tested myself. My mitochondrial DNA Haplogroup (which is also Charlotte’s) is…
T2b. As well, I’m missing a mutation in the HVR2 region, which is the part tested with the mtDNA Plus test, and I have an extra mutation in the Coding Region, which is the part tested in the Full Sequence test. Note that I have a few other extra mutations, but when I did the Haplogroup Analysis at this site, it indicated that those mutations are on markers that occur too frequently or change too frequently to be relevant for determining ancestry, so they can safely be ignored.
T2 is a European Haplogroup – which was no surprise to me. While not as prominent as Haplogroup H, which is found in about 44% of the British population, it’s not a rare one either. About 6% of the population of England is Haplogroup T2. It’s more predominant in the east of England. You can find more information on Haplogroup T2, along with a nice distribution map, at this site.
Theory 1: Mary Flintoff (possible mother) – Results
For Theory 1, I wasn’t able to get a descendant of Mary Flintoff to test, but a descendant of her sister Jane did agree. Pam’s results came back as…
U5. No match.
So unless there’s a misattributed parentage somewhere, most of the people who have parents for Charlotte on Ancestry have it wrong. And since I’m also a descendant of Mary Flintoff’s son Joseph Richardson, it also means that I had two ancestors with the same last name, who lived in the same area, born 3 years apart, who were not siblings, and likely not related at all!
Theory 2: Barbara Richardson (possible sister) – Results
So what about Theory 2? My tester for Theory 2 was Don, a descendant of Barbara Richardson. Don’s initial results came back as….
T2! As well, he was missing the same mutation I was, so that was a promising sign. But since the Coding Region is only tested with the Full Sequence test, the results were not yet conclusive. As I mentioned earlier, 6 percent of the British population are T2. And it’s even higher in other parts of Europe, so we could be the same haplogroup without having a recent common ancestor.
I ordered the upgrade, and waited again. Fortunately, those results didn’t take long to process. A couple of weeks later, I got notification that his full sequence results were in.
Well would you look at that! Don is also T2b. He also has the same extra mutation that I have. Plus, he has another one. What does that mean? Are we a match, or not?
To put these results in context, let’s look at all of my matches
As of today, I have 539 matches. Of those, 191 are at a genetic distance of 2 (meaning that we have two differences) and 340 are a genetic distance of 3 (meaning we have 3 differences). I have a common ancestor with all these people, but it could be REALLY far back.
I have only one perfect match (genetic distance of 0) – a woman named Alana from New Zealand. We are both T2b, plus we’re missing the same mutation (C195T) and we have the same extra mutation (A7374G). Her most distant known ancestor on her maternal line was born in 1796 (20 years after Charlotte) in West Sussex, England.
I also have seven matches with a genetic distance of 1, some of whom I’ve been in contact with. Six of the seven are all GD0 to each other. They are all missing the C195T mutation, but none of them have the A7374G mutation that Alana and I have. So again, we definitely have a common ancestor, but it’s hard to say how far back. That all depends on how long ago our 7374 changed from an A to a G.
And then there’s Don. He’s also a genetic distance of 1 to me, but for a different reason than the the other six people at GD1. Don is genetic distance 1 to me because he has an extra mutation that I don’t have.
Do you notice anything odd about Don’s extra mutation? It ends with a “D”. One of the first things I learned about DNA is that it can be one of 4 letters – A, T, C, or G. So what does “D” mean, you may ask? I know I sure did! That’s what’s called a “heteroplasmy”
[Note: See Debbie Kennett’s comment – Don’s extra mutation is a deletion, not a heteroplasmy. I’ll update soon with the correct information]
When they test your sample, they test many different cells. Usually, each of the cells has the same result for the same gene. But sometimes, it does not.
For my purposes, the most interesting bit about Don’s mutation being a heteroplasmy is that a heteroplasmy is usually a recent mutation – within the past 3 to 5 generations. Since our common ancestor would be 6 generations back for Don, it is most likely that his mutation occurred more recently than that, and our suspected common ancestor is indeed our common ancestor.
Possible outcomes revisited
In the first post in this series, I identified four possible outcomes:
A match between Don and I indicates that Charlotte Richardson and Barbara Richardson were sisters (or another maternal relationship, but given they have the same last name, sisters is the most likely relationship). And unless their parents were on Prince Edward Island, left and then returned, which is unlikely, we’re looking for parents who were on PEI, probably in Charlottetown, for at least a 6 years span – 1776 to 1782.
The question I most wanted to answer – who were Charlotte Richardson’s parents – remains unanswered. But knowing that Charlotte and Barbara were sisters gives us some very important clues.
In the fifth and final post of this series, I will discuss what this discovery has meant for the search for Charlotte’s parents, and give some final thoughts on the use of mtDNA for genealogy.
This is the third post of the series. In the first post, I introduced my 5th great grandmother, Charlotte Richardson and the two competing theories as to who her parents were. In the second post, I outlined the process I followed to identify possible matches, in order to test the two theories.
Now came the hard part – contacting people and getting them to agree to test. I tackled this in multiple ways, with help from Arthur Owen, another descendant of Charlotte Richardson and the person who first got me interested in genetic genealogy.
Get the word out
The first thing we did was to try to get the word out. Even before I had completed Step 1, Arthur spread the word through mailing lists, research groups and forums. I put a note on the WikiTree profiles of Charlotte Richardson, Mary Flintoff, Jane Flintoff and Barbara Richardson, stating that I was looking for female-line descendants to test. We had a few bites, but they were all from people who were descendants but not along an all-female line. Some of them offered to pass the information along to people who would be candidates, but nothing materialized. I’d still recommend doing this though, as you never know who you might reach this way.
Another way of reaching people was through cousin bait. I mentioned in the last post that one of the reasons I used WikiTree to document my research is because it’s excellent cousin bait. Sure enough, just a few days after I created the profile of one of Barbara Richardson’s descendants, I was contacted by a woman named Dana. She had been doing genealogy research and had googled her great-grandmother’s name and came upon the profile I had created. Dana contacted me to see if I could answer a question she had. While I couldn’t, I did take the opportunity to tell her why I had created the profile in the first place. While she was not a suitable candidate for my research, as it was her father’s grandmother, he was a direct female-line descendant of Barbara Richardson. Dana put me in touch with her father, Don, who kindly agreed to do the DNA test. Yay! I had my first tester!
The third way I reached people was through direct contact. For this, I used Ancestry. I figured I’d have better luck finding someone to test by reaching people who at least had an interest in genealogy than by emailing people whose names I had read in an obituary, though I was prepared to go there if I had to.
Once I identified some potential matches, I did a search in the Public Member Trees of Ancestry for the most recently deceased person in that line. I looked for people who had a lot of detail. I figured if someone didn’t know the name of a person’s spouse or where they were born, they were probably a more distant cousin and not a direct descendant. For those that did, I looked at the tree, to see if the member was indeed directly descended from the person I searched.
To each of these potential matches, I wrote a short, carefully crafted message, explaining who I was, what I was trying to accomplish and how they were connected. I offered to pay for the test and to provide any additional information they requested.
Most of people that I contacted never responded. But a full two months after I sent one query, I got a positive response, and I was in business! I now had two testers – one representing each theory.
There are two mtDNA tests available at Family Tree DNA (FTDNA), called mtDNA Plus and mtFull Sequence. Note that these are the prices at the time I’m writing this post (January 2018) – they do change periodically. And there’s usually a sale around Mother’s Day, so if you’re not in a rush to test, it might be worth waiting for a sale.
Prior to testing Pam and Don, I had already taken the mtDNA Full Sequence test and knew my haplogroup to be T2b, with some extra mutations (more on this in the next post). For comparison purposes, I started with the cheaper mtDNA Plus test for Pam and Don. This would be sufficient to rule out a match if, for example, their haplogroup was anything other than T. And if I had a match at that level, I could always upgrade to the full sequence test to confirm it.
Tests were ordered, cheeks were swabbed and finally, after months of research and reaching out to numerous people, all I had to do was wait for results. And as you’ll see in the next post, it was worth the wait.
In the first post of this series, I introduced my 5th great grandmother, Charlotte Richardson and the two competing theories as to who her parents were. In order to test those theories, I first had to identify some possible matches.
Follow the daughters
In order to find people who carry the mtDNA of Mary Flintoff and Barbara Richardson, I followed as many lines as I could in order to increase my chances of finding some living descendants who would agree to be tested.
Mary Flintoff had one known daughter, Elizabeth. She had one daughter, who had two daughters. I pursued this line, but since there was only one line to follow and I didn’t want to put all my eggs in one basket, I also looked at Mary Flintoff’s sister, Jane, who would carry the same mitochondrial DNA (assuming, of course, they had the same mother). Jane had 2 daughters, who between them had 8 daughters. One of Jane’s daughters, Elizabeth Humphrey, married Charles Dixon Jr. They left Sackville, New Brunswick in September 1837 with their 7 youngest children for Kirtland, Ohio where they joined the Church of Jesus Christ of Latter Day Saints. This would prove to be the easiest line to follow, due to the excellent genealogical record-keeping of the Mormons.
For Theory 2, Barbara Richardson had 5 daughters, who had 16 daughters among them. There were lots of choices of lines to pursue there, so when one line would fizzle out, there were others to try.
For each daughter, I looked for the following:
marriage record or wedding announcement to identify married name
all censuses following marriage to identify children
birth records of each daughter to confirm mother’s maiden name
marriage record or wedding announcement of each daughter to identify married name
death record and/or obituary
I worked systematically through each daughter and each daughter’s daughter until I got as far as I could go, then I’d go back a generation and start again with the next one.
Use online sources
While following the lines to present day, I used readily-available online sources. I used some Ancestry trees for hints, but I didn’t want to end up testing someone who wasn’t actually a descendant due to shaky trees. I started with the easy-to-access sources and figured I would dig deeper if I got stuck. Fortunately, I was able to get to present day on several lines using only online sources, through Family Search, Ancestry, Nova Scotia and New Brunswick Archives online, Find a Grave and Google searches, which were especially useful for recent obituaries.
Doing this, I discovered that it’s much easier to get to living descendants on lines that went to the US than on those that stayed in Canada. That 1940 census is very helpful when you’re trying to get to present day!
Throughout the process, I documented everything, including the sources I used. I created WikiTree profiles for each person, for a few reasons – First off, WikiTree is what I use for my own family research, so I’m used to it and I believe in the mandate of a collaborative family tree. I figured I may as well make this research available for others to benefit from and add to. As well, there was always the possibility that I would connect to trees already on WikiTree, which would save me some research. I did connect a few times to existing trees, but not on the lines I was interested in. But most importantly, WikiTree is great cousin bait. Since each profile is its own web page, when you search for a name in Google, the WikiTree profile will show up as a hit. And as you’ll see later, this would prove to come in very handy.
In addition to documenting on WikiTree, I also kept a running list in Evernote of where I was, who I had reviewed, and which lines had ended.
Lines ended for a few reasons:
On some, sources I checked had conflicting evidence. I didn’t want to spend a whole lot of time going down the line of someone who might not even be related.
Some people had no children, so their line ended
Others only had sons. Since only women pass on their mitochondrial DNA, men were not going to be much help (except for living men, as they have their mother’s mitochondrial DNA)
Some people I lost track of. I had several avenues to pursue, so I didn’t waste time trying to find people who couldn’t easily be found, but I knew I could always go back to them if I needed to.
When I came upon a possible contact, I added a checkbox beside their name. Once I had contacted them, I checked the box.
One I worked through the lines, I had about 10 possible living female-line descendants identified. Next came the fun part – making contact and asking people to give a cheek swab for a total stranger! I’ll cover that in the next post.